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Fig. 4 | Molecular Horticulture

Fig. 4

From: PeNAC67-PeKAN2-PeSCL23 and B-class MADS-box transcription factors synergistically regulate the specialization process from petal to lip in Phalaenopsis equestris

Fig. 4

Analyses of interactions of PeNAC67 and PeSCL23 with PeKAN2. a Subcellular localization of PeNAC67-GFP, PeSCL23-GFP and PeKAN2-GFP proteins in Nicotiana benthamiana leaf epidermal cells with DAPI staining. Scale bars = 50 μm. b Yeast two hybrid assay of PeNAC67 and PeSCL23 with PeKAN2(1–390). c and (d) BiFC assay was introduced to detect the interaction PeNAC67 and PeSCL23 with PeKAN2 in tobacco leaves. e and (f) Co-IP was used to detect the interaction between PeNAC67-Flag and PeSCL23-Myc with PeKAN2-HA proteins transiently expressed in tobacco leaves

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