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Fig. 3 | Molecular Horticulture

Fig. 3

From: PeNAC67-PeKAN2-PeSCL23 and B-class MADS-box transcription factors synergistically regulate the specialization process from petal to lip in Phalaenopsis equestris

Fig. 3

Phenotypes of PeNAC67 and PeSCL23 silencing lines and interaction between PeNAC67 with PeMADS3. a and (c) Mock and VIGS-treated lines with silenced expression of PeNAC67 (a) and PeSCL23 (c). The red arrows in (a) and (c) represent the part where the mutation occurs after VIGS treatment, and the blue arrows in (a) and (c) represent the corresponding part in the Mock. b and (d) Relative transcript levels of PeNAC67, PeMADS3 and PeMADS9 in Mock and VIGS-treated lines (b) and relative transcript levels of PeSCL23, PeMADS3 and PeMADS9 in Mock and VIGS-treated lines (d), asterisk in (b) and (d) indicated significant differences compared with the control, with one asterisk indicating P < 0.05 and two asterisks indicating P < 0.01. e Yeast two hybrid assay between PeNAC67 and PeMADS3/PeMADS9. f Co-IP was used to detect the interaction between PeNAC67-Flag and PeMADS3-Myc proteins transiently expressed in tobacco leaves. g Bimolecular fluorescence complementation (BiFC) assay was introduced to detect the interaction between PeNAC67 and PeMADS3 in tobacco leaves with DAPI staining. h Yeast two hybrid assay of PeSCL23 with B-class MADS-box protein

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Molecular Horticulture

ISSN: 2730-9401

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